10.6084/m9.figshare.8075564.v1
Ryu Nakata
Ryu
Nakata
Naoko Yoshinaga
Naoko
Yoshinaga
Masayoshi Teraishi
Masayoshi
Teraishi
Yutaka Okumoto
Yutaka
Okumoto
Naoki Mori
Naoki
Mori
An easy, inexpensive, and sensitive method for the quantification of chitin in insect peritrophic membrane by image processing
Taylor & Francis Group
2019
Chitin
chitosan
peritrophic membrane
image processing
2019-05-03 12:00:55
Presentation
https://tandf.figshare.com/articles/presentation/An_easy_inexpensive_and_sensitive_method_for_the_quantification_of_chitin_in_insect_peritrophic_membrane_by_image_processing/8075564
<p>Chitin, poly (β-(1→4)-<i>N</i>-acetyl-d-glucosamine), is an important biopolymer for insects that is utilized as a major component of peritrophic membrane. The chitin content in peritrophic membrane is of expedient interest from a pest control perspective, although it is hard to quantify chitin. In this study, we establish a facile method for the quantification of chitin in peritrophic membrane by image processing. In this method, chitin was indirectly quantified using chitosan–I<sub>3</sub><sup>−</sup> complex, which exhibited a specific red-purple color. A calibration curve using a chitosan solution showed good linearity in a concentration range of 0.05–0.5 μg/μL. We quantified the amount of chitin in peritrophic membrane of <i>Spodoptera litura</i> (Lepidoptera: Noctuidae) larvae using this method. Throughout the study, only common inexpensive regents and easily attainable apparatuses were employed. This method can be easily applied to the sensitive quantification of the amounts of chitin and chitosan in materials by wide range of researchers.</p> <p>Abbreviations: LOD: limit of detection; LOQ: limit of quantification; ROI: region of interest; RSD: relative standard deviation.</p> <p>Chitin in peritrophic membrane of insects was quantified by image processing with an</p> <p>easy, inexpensive and sensitive method.</p>