10.6084/m9.figshare.8095232.v1
Eda Yalcin
Eda
Yalcin
Goknur Kara
Goknur
Kara
Ekin Celik
Ekin
Celik
Ferda Alpaslan Pinarli
Ferda Alpaslan
Pinarli
Guleser Saylam
Guleser
Saylam
Ceren Sucularli
Ceren
Sucularli
Serhat Ozturk
Serhat
Ozturk
Esin Yilmaz
Esin
Yilmaz
Omer Bayir
Omer
Bayir
Mehmet Hakan Korkmaz
Mehmet Hakan
Korkmaz
Emir Baki Denkbas
Emir Baki
Denkbas
Preparation and characterization of novel albumin-sericin nanoparticles as siRNA delivery vehicle for laryngeal cancer treatment
Taylor & Francis Group
2019
Albumin
sericin
nanocarrier
siRNA
laryngeal cancer treatment
2019-05-08 14:03:32
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Preparation_and_characterization_of_novel_albumin-sericin_nanoparticles_as_siRNA_delivery_vehicle_for_laryngeal_cancer_treatment/8095232
<p>Small interfering RNA (siRNA)-based gene silencing strategy has high potential on suppressing specific molecular targets, involved in cancer progression. However, the lack of an effective nanocarrier system that safely delivers siRNA to its target still limits the clinical applications of siRNA. This study aimed to develop albumin-sericin nanoparticles (Alb-Ser NPs) as a novel siRNA delivery system for laryngeal cancer treatment. Nanoparticle formulations composed of albumin and sericin at different ratios (1:1, 2:1, 1:2 <i>w/w</i>) were synthesized by desolvation method. The nanoparticles were modified with poly-L-lysine (PLL) for siRNA binding and decorated with hyaluronic acid (HA) to target laryngeal cancer cell line, Hep-2. HA/PLL/Alb-Ser NPs were individually loaded with siRNAs for casein kinase 2 (CK2), Absent, Small, or Homeotic-Like (ASH2L), and Cyclin D1 genes, which are overexpressed in Hep-2 cells. Downregulation of genes was confirmed by real-time PCR (RT-PCR). Size, morphological, and thermogravimetric characterizations revealed that Alb-Ser NPs having 2:1 (<i>w/w</i>) ratio are the most optimized formulation. Between 36.8 and 61.3% of siRNA entrapment efficiencies were achieved. HA/PLL-siRNA/Alb-Ser (2:1) NPs-mediated gene silencing resulted in a significant inhibition of cell growth and induction of apoptosis in cells. Our findings showed that HA/PLL/Alb-Ser (2:1) NPs were promising as a siRNA carrier.</p>