Liu, Taiyu Zhao, Yixin Zhang, Jianguo Zhang, Jining Enhancement of xylanase expression by <i>Komagataella phaffii</i> through pexophagy inhibition <p>Xylanase is widely used in agriculture and food industry. To increase heterologous xylanase expression by <i>Komagataella phaffii</i> under the condition of mixed carbon source feeding, the <i>atg30</i> gene was knocked out from this yeast to inhibit pexophagy for increasing sorbitol consumption. <i>Komagataella phaffii atg30Δ</i> was constructed through homologous recombination that resulted in the inhibition of pexophagy. When compared with the control, the yield of secretory xylanase increased by 4.1 ± 0.2%, 5.7 ± 0.5%, 11.8 ± 0.4% and 11.4 ± 0.7% under the conditions of 1.5% methanol and 0.5, 1, 1.5 and 2% sorbitol addition, respectively. The residual sorbitol analysis showed that sorbitol consumed by <i>Komagataella phaffii atg30Δ</i> increased by 121.3 ± 9.3%, 66.8 ± 15.2% and 31.2 ± 3.2% following 1, 1.5 and 2 sorbitol addition, respectively. Thus, non-methanol carbon source, co-feeding with methanol, provided more energy without pexophagy by <i>atg30</i> gene knockout. Pexophagy inhibition increased the heterologous protein expression by <i>Komagataella phaffii</i>, which provided basis for increased yield of xylanase through pexophagy monitoring.</p> Gene knockout;Komagataella phaffii;mixed carbon source feeding;xylanase 2019-05-31
    https://tandf.figshare.com/articles/journal_contribution/Enhancement_of_xylanase_expression_by_i_Komagataella_phaffii_i_through_pexophagy_inhibition/8208290
10.6084/m9.figshare.8208290.v1