10.6084/m9.figshare.8268869.v1 Maja Karaman Maja Karaman Kristina Tesanovic Kristina Tesanovic Stanislava Gorjanovic Stanislava Gorjanovic Ferenc T. Pastor Ferenc T. Pastor Mladen Simonovic Mladen Simonovic Miodrag Glumac Miodrag Glumac Boris Pejin Boris Pejin Polarography as a technique of choice for the evaluation of total antioxidant activity: The case study of selected <i>Coprinus Comatus</i> extracts and quinic acid, their antidiabetic ingredient Taylor & Francis Group 2019 cyclic polyols quinic acid antioxidativity polarography HPMC assay 2019-06-13 12:34:35 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Polarography_as_a_technique_of_choice_for_the_evaluation_of_total_antioxidant_activity_The_case_study_of_selected_i_Coprinus_Comatus_i_extracts_and_quinic_acid_their_antidiabetic_ingredient/8268869 <p>This study was focused on <i>in vitro</i> screening of the total antioxidant activity of the selected extracts of the mushroom <i>Coprinus comatus</i> and quinic acid, one of their antidiabetic ingredients, by an uncommon electrochemical assay. Indeed, direct current (DC) polarographic HydroxoPerhydroxo Mercury(II) Complex (HPMC) assay based on decrease of anodic limiting current originating from HPMC formation in alkaline solutions of hydrogen peroxide at potential of mercury dissolution, observed upon gradual addition of antioxidants, was applied herein for the estimation of the natural products' antioxidativity. Quinic acid was found to exhibit most promising antioxidant potential (4.0 ± 0.2%µL<sup>−1</sup>) being ≈ 2-fold more active than the screened <i>C. comatus</i> extract samples. Actually, such a finding puts some light on the antioxidativity of cyclic polyols, well understimated class of organic compounds, compared to aromatic (poly)phenolics. As a low cost, easy-to handle and accurate this polarographic assay may be thoroughly recommended for much broader use. </p> <p></p>