Kumar Yadav, Vikash Shubhra Mandal, Rahul Lal Puniya, Bhanwar Singh, Sarman Yadav, Savita Studies on the interactions of SAP-1 (an N-terminal truncated form of cystatin S) with its binding partners by CD-spectroscopic and molecular docking methods <div><p>SAP-1 is a 113 amino acid long single-chain protein which belongs to the type 2 cystatin gene family. In our previous study, we have purified SAP-1 from human seminal plasma and observed its cross-class inhibitory property. At this time, we report the interaction of SAP-1 with diverse proteases and its binding partners by CD-spectroscopic and molecular docking methods. The circular dichroism (CD) spectroscopic studies demonstrate that the conformation of SAP-1 is changed after its complexation with proteases, and the alterations in protein secondary structure are quantitatively calculated with increase of α-helices and reduction of β-strand content. To get insight into the interactions between SAP-1 and proteases, we make an effort to model the three-dimensional structure of SAP-1 by molecular modeling and verify its stability and viability through molecular dynamics simulations and finally complexed with different proteases using ClusPro 2.0 Server. A high degree of shape complementarity is examined within the complexes, stabilized by a number of hydrogen bonds (HBs) and hydrophobic interactions. Using HB analyses in different protein complexes, we have identified a series of key residues that may be involved in the interactions between SAP-1 and proteases. These findings will assist to understand the mechanism of inhibition of SAP-1 for different proteases and provide intimation for further research.</p></div> protease;hb;ClusPro 2.0 Server;type 2 cystatin gene family;binding partners;interaction;docking methods SAP 2015-10-08
    https://tandf.figshare.com/articles/journal_contribution/Studies_on_the_interactions_of_SAP_1_an_N_terminal_truncated_form_of_cystatin_S_with_its_binding_partners_by_CD_spectroscopic_and_molecular_docking_methods/858854
10.6084/m9.figshare.858854.v8