10.6084/m9.figshare.8851424.v2 Shengnan Tang Shengnan Tang Aiping Wang Aiping Wang Xiuju Yan Xiuju Yan Liuxiang Chu Liuxiang Chu Xiucheng Yang Xiucheng Yang Yina Song Yina Song Kaoxiang Sun Kaoxiang Sun Xin Yu Xin Yu Rongxia Liu Rongxia Liu Zimei Wu Zimei Wu Peng Xue Peng Xue Brain-targeted intranasal delivery of dopamine with borneol and lactoferrin co-modified nanoparticles for treating Parkinson’s disease Taylor & Francis Group 2020 Dopamine lactoferrin borneol nose-to-brain targeted nanoparticles Parkinson’s disease 2020-01-13 16:03:22 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Brain-targeted_intranasal_delivery_of_dopamine_with_borneol_and_lactoferrin_co-modified_nanoparticles_for_treating_Parkinson_s_disease/8851424 <p>Efficient delivery of brain-targeted drugs is highly important for successful therapy in Parkinson’s disease (PD). This study was designed to formulate borneol and lactoferrin co-modified nanoparticles (Lf-BNPs) encapsulated dopamine as a novel drug delivery system to achieve maximum therapeutic efficacy and reduce side effects for PD. Dopamine Lf-BNPs were prepared using the double emulsion solvent evaporation method and evaluated for physicochemical and pharmaceutical properties. <i>In vitro</i> cytotoxicity studies indicated that treatment with dopamine Lf-BNPs has relatively low cytotoxicity in SH-SY5Y and 16HBE cells. Qualitative and quantitative cellular uptake experiments indicated that Lf modification of NPs increased cellular uptake of SH-SY5Y cells and 16HBE cells, and borneol modification can promote the cellular uptake of 16HBE. <i>In vivo</i> pharmacokinetic studies indicated that AUC<sub>0–12 h</sub> in the rat brain for dopamine Lf-BNPs was significantly higher (<i>p</i> < .05) than that of dopamine nanoparticles. Intranasal administration of dopamine Lf-BNPs effectively alleviated the 6-hydroxydopamine-induced striatum lesion in rats as indicated by the contralateral rotation behavior test and results for striatal monoamine neurotransmitter content detection. Taken together, intranasal administration of dopamine Lf-BNPs may be an effective drug delivery system for Parkinson’s disease.</p>