%0 Generic %A Balakrishna, Konduru %A Sreerohini, Sagi %A Parida, Manmohan %D 2019 %T Ready-to-use single tube quadruplex PCR for differential identification of mutton, chicken, pork and beef in processed meat samples %U https://tandf.figshare.com/articles/dataset/Ready-to-use_single_tube_quadruplex_PCR_for_differential_identification_of_mutton_chicken_pork_and_beef_in_processed_meat_samples/8870600 %R 10.6084/m9.figshare.8870600.v1 %2 https://tandf.figshare.com/ndownloader/files/16263764 %2 https://tandf.figshare.com/ndownloader/files/16263767 %2 https://tandf.figshare.com/ndownloader/files/16263770 %2 https://tandf.figshare.com/ndownloader/files/16263773 %K Quadruplex PCR %K cryopreservation %K trehalose %K adulteration %K meat %X

A reliable and sensitive identification method is required to tackle food adulteration mainly in meat production. We developed a dry reagent based ready-to-use single tube quadruplex PCR assay for accurate identification of chicken, mutton, beef and pork. The assay was found to be specific and reproducible. Thermo-stability studies of lyophilized PCR master mix were conducted at different temperature and time intervals, which revealed significant stability for 75 days at 4°C and for 60 days at 25°C. The developed assay was shown to be sensitive down to 16 pg DNA per reaction and the detection limit was found to be 0.01% (w/w) of each species. Furthermore, this method has been applied to the analysis of 68 commercial meat products and the results indicated that nine samples contained non-declared meat components. This dry reagent-based quadruplex PCR assay can be utilized to monitor various processed food products and also to maintain quality control in food industries mainly in the resource-limited settings.

%I Taylor & Francis