Oliver, Clara Hernández, Iker Caminal, Marta Lara, José M. Fernàndez, Carolina <i>Pseudomonas putida</i> strain B2017 produced as technical grade active ingredient controls fungal and bacterial crop diseases <p>Biological control is emerging as a feasible alternative to chemical pesticides for the protection of crop plants. <i>Pseudomonas putida</i> shows enormous potential as biological control agent (BCA) not only because it is well reported to control a number of relevant crop diseases, but also because it has a short generation time, colonises plants and their environment, exerts its BCA activity through a range of different mechanisms, and promotes plant growth. Despite of these advantages no plant protection product based on <i>P. putida</i> is available in the market so far. In the present manuscript we describe the production of <i>P. putida</i> B2017, a novel biofungicide and biobactericide strain, at increasing scale – from laboratory (Erlenmeyer flasks) to pilot scale (125-L bioreactors) – as part of development process of a new commercial plant protection product. The technical grade active ingredient (TGAI) produced, regardless of the production scale, is as effective as the chemical reference against <i>Fusarium oxysporum f.sp. radicis-lycopersici</i> in tomato, <i>Rhizoctonia solani</i> in potato, <i>Sclerotinia sclerotiorum</i> in lettuce and <i>Pectobacterium atrosepticum</i> in potato. The TGAI titration obtained in 125-L bioreactors is high enough as to warrant effective TGAI concentration in a putative end-product even after typical viability losses expected during formulation. In summary, we hereby show that <i>P. putida</i> B2017 is a promising BCA not only because its efficacy against plant pathogens, but also because it can be produce at large scale.</p> Biological control;crop diseases;Pseudomonas putida B2017;technical grade active ingredient;scale-up 2019-07-19
    https://tandf.figshare.com/articles/journal_contribution/_i_Pseudomonas_putida_i_strain_B2017_produced_as_technical_grade_active_ingredient_controls_fungal_and_bacterial_crop_diseases/8959505
10.6084/m9.figshare.8959505.v1