10.6084/m9.figshare.9119939.v1 Munther Alomari Munther Alomari B. Rabindran Jermy B. Rabindran Jermy Vijaya Ravinayagam Vijaya Ravinayagam Sultan Akhtar Sultan Akhtar Sarah Ameen Almofty Sarah Ameen Almofty Suriya Rehman Suriya Rehman Hiba Bahmdan Hiba Bahmdan Sayed AbdulAzeez Sayed AbdulAzeez J. Francis Borgio J. Francis Borgio Cisplatin-functionalized three-dimensional magnetic SBA-16 for treating breast cancer cells (MCF-7) Taylor & Francis Group 2019 SPIONs cisplatin SBA-16 polyacrylic acid nanotherapeutics cancer therapy MCF-7 HFF-1 TEM 2019-07-27 07:20:19 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Cisplatin-functionalized_three-dimensional_magnetic_SBA-16_for_treating_breast_cancer_cells_MCF-7_/9119939 <p>The engineering of multifunctional therapeutics in an integrated single platform is demonstrated using three-dimensional SBA-16 (S-16). 10 wt% iron oxide nanoparticles (Fe) were loaded into the cage type of cubic pores through enforced adsorption technique. Fe/S-16 is then functionalized with amine-based silane (A), polyacrylic acid (P) and cisplatin (Cp). The physicochemical textural analysis showed the formation of nano metal oxide distributions at pore walls of S-16 with magnetization of 2.39 emu/g. S-16 based nanoformulations showed high percentage of Cp adsorption (90%) and percentage cumulative release (60%). <i>in vitro</i> study of Fe/S-16-A-Cp showed high toxicity against breast cancer cell line MCF-7 and normal cell line Human foreskin fibroblast (HFF-1) compared to Fe/S-16 indicating cisplatin profusion inside the cells than free cisplatin. While skin fibroblast seems to be resistant to Fe/S-16-AP-Cp with very high LC<sub>50</sub> in compare to MCF-7. This indicates the unrelease of cisplatin in skin fibroblast after Fe/S-16-AP-Cp treatment due to effective encapsulation inside the cubic pores and core blockage due to pH-sensitive polyacrylic acid. Also, these treatments resulted in morphological changes in the cells such as DNA condensation and nuclear fragmentation.</p>