RACK1 affects the progress of G2/M by regulating Aurora-A
Suqin Shen
Huan Feng
Yichen Le
Jun Ni
Long Yu
Jiaxue Wu
Meirong Bai
10.6084/m9.figshare.9163967.v1
https://tandf.figshare.com/articles/journal_contribution/RACK1_affects_the_progress_of_G2_M_by_regulating_Aurora-A/9163967
<p>Aurora-A is a serine/threonine kinase, which is overexpressed in multiple human cancers and plays a key role in tumorigenesis and tumor development. In this study, we found that the receptor of activated C-kinase1 (RACK1), an important regulator of biological functions, interacted with Aurora-A and co-localized with Aurora-A at centrosomes. Moreover, RACK1 induces the auto-phosphorylation of Aurora-A in vitro and in vivo. Depletion of RACK1 impaired the activation of Aurora-A in late G2 phase, then inhibited the mitotic entry and leaded to multi-polarity, severe chromosome alignment defects, or centrosome amplification. Taken together, these results suggest that RACK1 is a new partner of Aurora-A and play a critical role in the regulation of the Aurora-A activity during mitosis, which may provide a basis for future anticancer studies targeting Aurora-A.</p>
2019-07-30 05:00:33
Mitosis
Aurora-A
RACK1
Phosphorylation
G2/M