10.6084/m9.figshare.9192620.v1
Naoki Yamamoto
Naoki
Yamamoto
Takehiro Masumura
Takehiro
Masumura
Kentaro Yano
Kentaro
Yano
Toshio Sugimoto
Toshio
Sugimoto
Pattern analysis suggests that phospho<i>enol</i>pyruvate carboxylase in maturing soybean seeds promotes the accumulation of protein
Taylor & Francis Group
2019
Anaplerotic
principal component analysis
phosphoenolpyruvate carboxylase
seed protein
soybean
2019-07-31 08:11:25
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Pattern_analysis_suggests_that_phospho_i_enol_i_pyruvate_carboxylase_in_maturing_soybean_seeds_promotes_the_accumulation_of_protein/9192620
<p>The protein and oil contents in soybean seeds are major factors in seed quality. Seed proteins and oils are synthesized from sucrose and nitrogenous compounds transported into maturing seeds. In this study, we compared changes in the activity of phospho<i>enol</i>pyruvate carboxylase (PEPC) and the accumulation profiles of protein and oil in maturing seeds of two soybean cultivars, which exhibit different protein and oil contents in seeds, to determine the interrelationships of them. A principal component analysis indicated a concordance of seed PEPC activity with the protein content, but did not with the oil content. PEPC activity per seed was highest in the late maturation stage, when the physiological status of the vegetative organs drastically changed. The high-protein cultivar had higher PEPC activity compared to the low-protein cultivar. These results highlight the biological role of PEPC in the synthesis of protein, therefore it was implied that PEPC could be a biomarker in soybean breeding.</p> <p><b>Abbreviations:</b> ANOVA: analysis of variance; DS: developmental stage; DW: dry weight; FW: fresh weight; NIR: near infrared; PEP(C): phospho<i>enol</i>pyruvate (carboxylase); PC(A): principal component (analysis); S.E.: standard error; WC: water content.</p> <p>Soy seed PEPC play a role for providing carbon skelton of amino acids of storage protein from starch in responce to N supply at the late maturing stage.</p>