Li, Weijie Yan, Ruicong Liu, Yong He, Chuanchuan Zhang, Xiaojuan Lu, Yao Khan, Muhammad Waseem Xu, Chuanrui Yang, Tan Xiang, Guangya Co-delivery of Bmi1 small interfering RNA with ursolic acid by folate receptor-targeted cationic liposomes enhances anti-tumor activity of ursolic acid <i>in vitro</i> and <i>in vivo</i> <p>Overexpression of Bmi1 gene is an important feature of cancer stem cell in various human tumors. Therefore, Bmi1 gene can be a potential target for small interfering RNA (siRNA) mediated cancer therapy. Ursolic acid (UA) as a natural product plays a pivotal role in anti-tumor field, although its performance is limited by low bioavailability and poor hydrophilicity. A folate receptor-targeted cationic liposome system was designed for the purpose of investigating the relationship between Bmil siRNA and UA. The folate receptor-targeted cationic liposomes co-delivering UA and Bmi1 siRNA (FA-UA/siRNA-L) were fabricated by electrostatic interaction between folate UA liposome (FA-UA-L) and Bmi1 siRNA. Tumor growth is inhibited by FA-UA/siRNA-L <i>in vitro</i> and <i>in vivo</i> and this inhibition is contributed by a synergistic anti-tumor effect of UA and Bmi1 siRNA. The western blot measurement of apoptosis-protein and cancer stem cell (CSC) marked-protein demonstrated that UA led to activation-induced tumor cell death and Bmi1 siRNA resulted in inhibition of cancer stem cells. Overall, these results indicate that Bmi1 as a regulating gene for cancer stem cell is an effective target for cancer treatment using siRNA and co-delivery of UA and Bmi1 siRNA using folate-targeted liposomes is a promising strategy for improved anti-tumor effect.</p> Liposome;Bmi1 siRNA;ursolic acid;folate receptor;tumor targeting 2019-08-01
    https://tandf.figshare.com/articles/journal_contribution/Co-delivery_of_Bmi1_small_interfering_RNA_with_ursolic_acid_by_folate_receptor-targeted_cationic_liposomes_enhances_anti-tumor_activity_of_ursolic_acid_i_in_vitro_i_and_i_in_vivo_i_/9204926
10.6084/m9.figshare.9204926.v1