10.6084/m9.figshare.9604376.v1 Xue Gong Xue Gong Yan Xu Yan Xu Kai Ren Kai Ren Xiaorong Bai Xiaorong Bai Chunhong Zhang Chunhong Zhang Minhui Li Minhui Li Phenylethanoid glycosides from <i>Paraboea martinii</i> protect rat pheochromocytoma (PC12) cells from hydrogen peroxide-induced cell injury Taylor & Francis Group 2019 Paraboea martinii phenylethanoid glycosides neuroprotective effects zinc protoporphyrin HO-1 2019-08-14 07:45:48 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Phenylethanoid_glycosides_from_i_Paraboea_martinii_i_protect_rat_pheochromocytoma_PC12_cells_from_hydrogen_peroxide-induced_cell_injury/9604376 <p>In this study, we isolated eight phenylethanoid glycosides from <i>Paraboea martinii</i> for the first time, and evaluated the mechanism underlying their neuroprotective effects against H<sub>2</sub>O<sub>2</sub>-induced injury in PC12 cells. The MTS method was utilized to screen the phenylethanoid glycosides for protective ability. Next, qRT-PCR and western blotting analysis were used to detect the transcription levels of HO-1 and GCLC, which are regulated by Nrf2. The inhibitor ZnPP was used to analyze the involvement of Nrf2 in HO-1 expression. Analyses showed that caleolarioside B, paraboside B, and paraboside II also upregulated the expression of HO-1, but showed no obvious effect on GCLC. Pretreatment with ZnPP significantly reduced the neuroprotective effects. Thus, phenylethanoid glycosides isolated from <i>P. martinii</i> protected PC12 cells from H<sub>2</sub>O<sub>2</sub>-induced damage by upregulating HO-1. The results provided evidence that <i>P. martinii</i> might be a potential therapeutic agent for the treatment of neurodegenerative diseases.</p> <p>Phenylethanoid glycosides was isolated from <i>P. martinii</i> could protect PC12 cells from.</p>