%0 Journal Article %A Gong, Xue %A Xu, Yan %A Ren, Kai %A Bai, Xiaorong %A Zhang, Chunhong %A Li, Minhui %D 2019 %T Phenylethanoid glycosides from Paraboea martinii protect rat pheochromocytoma (PC12) cells from hydrogen peroxide-induced cell injury %U https://tandf.figshare.com/articles/journal_contribution/Phenylethanoid_glycosides_from_i_Paraboea_martinii_i_protect_rat_pheochromocytoma_PC12_cells_from_hydrogen_peroxide-induced_cell_injury/9604376 %R 10.6084/m9.figshare.9604376.v1 %2 https://tandf.figshare.com/ndownloader/files/17250455 %K Paraboea martinii %K phenylethanoid glycosides %K neuroprotective effects %K zinc protoporphyrin %K HO-1 %X

In this study, we isolated eight phenylethanoid glycosides from Paraboea martinii for the first time, and evaluated the mechanism underlying their neuroprotective effects against H2O2-induced injury in PC12 cells. The MTS method was utilized to screen the phenylethanoid glycosides for protective ability. Next, qRT-PCR and western blotting analysis were used to detect the transcription levels of HO-1 and GCLC, which are regulated by Nrf2. The inhibitor ZnPP was used to analyze the involvement of Nrf2 in HO-1 expression. Analyses showed that caleolarioside B, paraboside B, and paraboside II also upregulated the expression of HO-1, but showed no obvious effect on GCLC. Pretreatment with ZnPP significantly reduced the neuroprotective effects. Thus, phenylethanoid glycosides isolated from P. martinii protected PC12 cells from H2O2-induced damage by upregulating HO-1. The results provided evidence that P. martinii might be a potential therapeutic agent for the treatment of neurodegenerative diseases.

Phenylethanoid glycosides was isolated from P. martinii could protect PC12 cells from.

%I Taylor & Francis