10.6084/m9.figshare.9609422.v1 Shen Sheng Shen Sheng Wang Jiwen Wang Jiwen Zhang Dexiang Zhang Dexiang Zheng Bohao Zheng Bohao Wang Yueqi Wang Yueqi Liu Han Liu Han Ni Xiaoling Ni Xiaoling Suo Tao Suo Tao Houbao Liu Houbao Liu DMBT1 suppresses progression of gallbladder carcinoma through PI3K/AKT signaling pathway by targeting PTEN Taylor & Francis Group 2019 Gallbladder carcinoma DMBT1 PTEN PI3K/AKT 2019-08-14 09:41:55 Dataset https://tandf.figshare.com/articles/dataset/DMBT1_suppresses_progression_of_gallbladder_carcinoma_through_PI3K_AKT_signaling_pathway_by_targeting_PTEN/9609422 <p>Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Deleted in malignant brain tumors 1 (DMBT1) is low-expression during cancer progression and as a potential tumor-suppressor gene in various types of cancer. However, its role in Gallbladder cancer remains poorly understood. Here, we found that DMBT1 was significantly low-expression and deletion of copy number in GBC tissues by qRT-PCR and Western blot. Overexpression of DMBT1 impaired survival, promoted apoptosis in GBC cells <i>in vitro</i>, and inhibited tumor progression <i>in vivo</i>. Further study of underlying mechanisms demonstrated that DMBT1 combined with PTEN which could stabilize PTEN protein, resulting in inhibiting the activation of PI3K/AKT signaling pathway. Our study revealed a new sight of DMBT1 as a tumor-suppressor gene on the PI3K/AKT pathway in GBC, which may be a potential therapeutic target for improving treatment.</p> <p>Down-regulation of DMBT1 suppresses progression of gallbladder carcinoma through PI3K/AKT signaling pathway activation by targeting PTEN. </p>