Anti-inflammatory and cytotoxic agents from <i>Xylaria</i> sp. SWUF09-62 fungus Theerawat Patjana Phongphan Jantaharn Praewpan Katrun Wiyada Mongkolthanaruk Nuttika Suwannasai Thanaset Senawong Sarawut Tontapha Vittaya Amornkitbumrung Sirirath McCloskey 10.6084/m9.figshare.9638447.v1 https://tandf.figshare.com/articles/journal_contribution/Anti-inflammatory_and_cytotoxic_agents_from_i_Xylaria_i_sp_SWUF09-62_fungus/9638447 <p>The ongoing search for anti-cancer agents from microorganisms led to the isolation of four new compounds including 6-ethyl-8-hydroxy-4<i>H</i>-chromen-4-one (<b>1</b>), 6-ethyl-7,8-dihydroxy-4<i>H</i>-chromen-4-one (<b>2</b>), (3<i>S</i>)-3,4-dihydro-8-hydroxy-7-methoxy-3-methylisocoumarin (<b>3</b>) and (3<i>S</i>)-3,4-dihydro-5,7,8-trihydroxy-3-methylisocoumarin (<b>4</b>), together with eleven known compounds (<b>5</b>–<b>15</b>) from <i>Xylaria</i> sp. SWUF09-62 fungus. The chemical structures were deduced from IR, 1D and 2D NMR, and MS data. The absolute configurations of <b>3</b> and <b>4</b> were determined by ECD experiment. Compounds <b>2</b> and <b>4</b> indicated possible chemo-prevention and chemo-therapeutic properties, exhibited anti-inflammatory properties by reducing nitric oxide production in LPS-stimulated RAW264.7 cells (IC<sub>50</sub> = 1.57 ± 0.25 and 3.02 ± 0.27 <i>μ</i>g/mL) and cytotoxicity against HT29 cells (IC<sub>50</sub> = 16.46 ± 0.48 and 97.78 ± 7.14 <i>μ</i>g/mL).</p> <p></p> 2019-08-16 11:06:55 Anti-inflammatory chromone cytotoxicity mellein nitric oxide (NO) Xylaria