A green approach to synthesize controllable silver nanostructures from <i>Limonia acidissima</i> for inactivation of pathogenic bacteria

<p>Controllable silver nanoparticles were developed by a green approach using extracts of both leaves and bark of <i>Limonia acidissima</i> tree. Due to the presence of phytochemical compounds in <i>L. acidissima</i> leaves and bark; such as saponins, phenolic compounds, phytosterols, and quinines present in extracts act as reductants, hence the silver nanoparticles were easily produced under mild conditions. The formation and kinetic study of silver nanoparticles were verified by UV–vis spectroscopy. Highly stable and uniform size silver nanoparticles were produced using bark extract reduction than leaf extract and confirmed by dynamic light scattering and transmission electron microscopy analysis. Further we applied antibacterial activity on both <i>Escherichia coli</i> and <i>Bacillus subtilis</i>. The results suggest that the silver nanoparticles suspension exhibits excellent antibacterial activity. The present study is a simple and eco-friendly approach for production of silver nanoparticles in the large scale up and could be easily commercialized, especially biological applications.</p>