A new eremophilane glycoside from the fruits of <i>Physalis pubescens</i> and its cytotoxic activity

<p><i>Physalis pubescens</i> L. has been used as a traditional folk medicine in China. Comprehensive studies aimed at identifying the bioactive constituents are still lacking. In the course of searching bioactive secondary metabolites from <i>P. pubescens</i> L., phytochemical investigation of the fruits of <i>P. pubescens</i> L. led to the isolation of one new eremophilane glycoside, 1<i>β</i>,3<i>α</i>-dihydroxy-7<i>α</i>-eremophila-911-dien-3-<i>O</i>-[<i>α</i>-L-arabinopyranosyl-(l→6)]-<i>β</i>-D-glucopyranoside (<b>1</b>), and six known glycosides (<b>2–7</b>). Their structures were established via extensive spectroscopic data including 1D and 2D NMR and HRESIMS. Cytotoxic effects against six human cancer cell lines (C4-2B, A375, A375-S2, 786-O, Caki-2, and ACHN) and one human normal hepatic cell line (L02) were evaluated and compounds <b>1</b> and <b>2</b> exhibited moderate cytotoxicity against C4-2B and A375 cell lines with IC<sub>50</sub> values in the range of 6.78–28.3 μM. The study indicates that the fruits of <i>P. pubescens</i> L. have the potential to be used for drug discovery.</p>