Aerosolization of Two Strains (Ice+ and Ice–) of <i>Pseudomonas syringae</i> in a Collison Nebulizer at Different Temperatures

<div><p>The aerosolization of microorganisms from aquatic environments is understudied. This article describes a study in which an ice nucleation active (ice+) strain and a nonice nucleation active (ice−) strain of the bacterium <i>Pseudomonas syringae</i> were aerosolized from aqueous suspensions under artificial laboratory conditions using a collison nebulizer. The aerosolization of <i>P. syringae</i> was not influenced by water temperatures between 5° and 30°C. In general, the culturability (viability) of <i>P. syringae</i> in aerosols increased with temperature between 5 and 30°C. The ice+ strain was aerosolized in greater numbers than the ice– strain at all temperatures studied, suggesting a possible connection between the ice nucleation phenotype and aerosol production. Together, our results suggest that <i>P. syringae</i> has the potential to be aerosolized from natural aquatic environments, such as streams, rivers, ponds, and lakes; known reservoirs of <i>P. syringae</i>. Future work is needed to elucidate the mechanisms of aerosolization of <i>P. syringae</i> from natural aquatic systems.</p><p>Copyright 2015 American Association for Aerosol Research</p></div>