Taylor & Francis Group
Browse
gnpl_a_1114941_sm4221.pdf (5.14 MB)

Antileishmanial diterpenoid alkaloids from Aconitum spicatum (Bruhl) Stapf

Download (0 kB)
journal contribution
posted on 2015-11-28, 00:00 authored by Sajan L. Shyaula, Tensangmu Tamang, Nida Ghouri, Achyut Adhikari, Shanti Marasini, Gan B. Bajracharya, Mangala D. Manandhar, Muhammad Iqbal Choudhary

The crude extracts of tubers of Aconitum spicatum (Bruhl) Stapf were investigated for in vitro antileishmanial activity against Leishmania major. The dichloromethane extract at pH 2.5 showed antileishmanial activity with IC50 value of 27.10 ± 0.0 μg/mL. Chromatographic purification of the dichloromethane extract led to isolation of three C-19 norditerpenoid alkaloids indaconitine (1), chasmaconitine (2) and ludaconitine (3). Compounds 3 and 2 showed antileishmanial activity with IC50 = 36.10 ± 3.4 and 56.30 ± 2.1 μg/mL, respectively. Compound 1 was less effective (IC50 > 100 μg/mL). The cytotoxicity of compounds 1, 2 and 3 studied against MCF7, HeLa and PC3 cancer cell lines and 3T3 normal fibroblast cell line did not show cytotoxicity at 30 μM.

History