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Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues

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posted on 09.01.2020 by Junko Ohki, Akihiko Sakashita, Eitaro Aihara, Akihiko Inaba, Hironobu Uchiyama, Masahito Matsumoto, Yuzo Ninomiya, Takumi Yamane, Yuichi Oishi, Ken Iwatsuki

Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.

Statistical analysis of genes preferentially expressed in Ngn3+ vs Ngn3- cells derived from intestinal organoids of Ngn3-EGFP mice.

Funding

This work was supported by the MEXT-Supported Program for the Strategic Research Foundation at Private Universities 2013–2017 (S1311017), JSPS KAKENHI (208920 to K.I., 5H02571 to Y.N.), The Japan Chemical Research Foundation (to K.I.), Grant-in-Aid for Advanced Research Project (to K.I.) and Grant for Ph.D. Research (to J.O.) from Tokyo University of Agriculture.

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