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D-Alanine Is Reduced by Ocular Hypertension in the Rat Retina

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posted on 2019-10-04, 07:27 authored by Takashi Kanamoto, Hiroaki Sakaue, Yasushi Kitaoka, Ryo Asaoka, Kei Tobiume, Yoshiaki Kiuchi

Purpose: The purpose of this study was to investigate the physiological changes of amino acids in the rat retina caused by ocular hypertension.

Methods: Adult Wistar rats were used as an experimental model of ocular hypertension. Retinas were hydrolyzed with HCl at 108°C to isolate amino acids. Residual amino acids were measured by reverse-phase high-performance liquid chromatography and the total volume of residual amino acids and the ratio of D- and L-amino acids were analyzed. Free D- and L-alanine levels were also measured using two-dimensional HPLC.

Results: The amount of retinal alanine decreased in ocular hypertension compared with the control (p < .05, Student’s t-test); the amounts of other amino acids did not differ between the two conditions.

The D/L ratio of alanine was higher than that of other amino acids. Ocular hypertension reduced the D/L ratio of retinal alanine, while that of other amino acids was unchanged. Ocular hypertension increased the D/L ratio of free alanine.

Conclusions: Ocular hypertension reduced the D/L ratio of retinal alanine, presumably due in large part to alanine peptides.

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