Taylor & Francis Group
Browse
tbbb_a_1670044_sm4252.docx (4.28 MB)

Effects of normothermic microwave irradiation on CD44+/CD24 in breast cancer MDA-MB-231 and MCF-7 cell lines

Download (4.28 MB)
journal contribution
posted on 2019-09-27, 11:54 authored by Mamiko Asano, Satoshi Tanaka, Minoru Sakaguchi

We previously reported that MDA-MB-231 and MCF-7 cells, which are breast cancer cell lines and have cancer and cancer-initiating cells (CICs), were killed following normothermic microwave irradiation in which the cellular temperature was maintained at 37°C. In this study, we investigated the percentages of live or dead cells among CD44+/CD24 cells, which were defined as CICs among MDA-MB-231 and MCF-7 cells, and other types of cells in response to microwave irradiation. CD44+/CD24 cells among MDA-MB-231 cells were killed, thereby decreasing the number of cells, whereas the number of live CD44+/CD24 MCF-7 cells was increased following microwave irradiation. Moreover, adhesion, invasion, and migration were decreased in MDA-MB-231 cells, and the activation of matrix metalloproteinase-2 (MMP-2) in MDA-MB-231 cells was increased following microwave irradiation. These decreased cell activities might have been caused by MMP-2 activation and population changes in CD44+/CD24 in MDA-MB-231 cells.

Abbreviations: APC: allophecocyanin; CBB: coomassie Brilliant Blue; CD: cluster of differentiation; CICs: cancer-initiating cells; FACS: fluorescence-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; FTDT: finite-difference time domain; HER2: human epidermal growth factor receptor type 2; PI: propidium iodide.

Functional changes of MDA-MB-231 cells by normothermic microwave irradiation.

Funding

Parts of this study were funded through the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 15K21516 and 18K12123, and the Exploratory Research on Humanosphere Science 2016 at the Research Institute for Sustainable Humanosphere, Kyoto University.Japan Society for the Promotion of Science [15K21516,18K12123]; the Exploratory Research on Humanosphere Science 2016 at the Research Institute for Sustainable Humanosphere.

History