Taylor & Francis Group
Browse
geac_a_1641202_sm9198.doc (9.44 MB)

Fluorescence- and FRET-based mercury (II) sensor

Download (9.44 MB)
journal contribution
posted on 2019-07-22, 11:32 authored by Jaba Saha, Sudip Suklabaidya, Jayasree Nath, Arpan Datta Roy, Bapi Dey, Dibyendu Dey, D. Bhattacharjee, Syed Arshad Hussain

A mercury (II) sensor has been proposed based on Fluorescence Resonance Energy Transfer (FRET) between N,N’-dioctadecyl thiacyanine perchlorate (NK) and Octadecyl rhodamine B chloride (RhB). Out of these two molecules, NK is sensitive to Hg2+ ions due to the presence of sulphur atom in it. Accordingly, presence of Hg2+ ions affects the NK fluorescence as well as FRET from NK to RhB. Our results showed that NK fluorescence intensity and FRET efficiency linearly decrease with an increase in Hg2+ ion concentration. With proper optimisation, present system under investigations can be used to sense Hg2+ ions in aqueous solution with a detection limit of 9.13 ppb. Advantage of this present system is that it is very simple compared to the other FRET-based system and also it works under aqueous environment. This method has also been tested using real lake water, and satisfactory results were obtained.

Funding

This work was supported by the DST, Govt. of India [EMR/2014/000234]; FIST DST project [SR/FST/PSI-191/2014]; UGC, Govt. of India [UGC – SAP program 2016]; DST-WOS-A project – Govt. of India [SR/WOS-A/PM-78/2016].

History