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Genome-wide assessment of population structure and genetic diversity for Anabasis aphylla based on specific length amplification fragment sequencing

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posted on 2020-04-09, 07:10 authored by Yaling Chang, Mengwen Peng, Guangming Chu, Mei Wang

Anabasis aphylla is a salt and alkali resistant shrub grows in arid and semi-arid areas, which is also a constructive species of desert soil. To reveal the population structure and genetic diversity in populations of A. aphylla, a total of 106 samples were used as experimental materials for sequencing by specific-locus amplified fragment sequencing. In total, we obtained 300105 SNP which had high consistency. Phylogenetic tree of A. aphylla result showed there was a certain difference between these samples, which can be divided into two groups. The Shanon–Wiener index of A. aphylla was 0.4440 and the Nei diversity index was 0.2854. AMOVA analysis showed there was genetic differentiation among the seven groups to a certain level. The genetic diversity analysis showed the genetic variation of A. aphylla mainly from groups. This study provides a reference for further comprehensive study of the genetic structure and evolutionary adaptation of A. aphylla.

Funding

This work was supported by the Program of the National Natural Science Foundation of China [grant number 31460187], General Financial Grant of the China Postdoctoral Science Foundation [grant number 2017M613253] and Scientific Research Foundation of Shihezi University for Advanced Talents [grant numbers RCZX201518, RCZX201521].

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