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Illumina midi-barcodes: quality proof and applications

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journal contribution
posted on 2019-04-06, 06:25 authored by Julia Lanner, Manuel Curto, Bärbel Pachinger, Ulrich Neumüller, Harald Meimberg

DNA barcoding constitutes a supplemental genetically based characterization tool for the identification of species. Traditionally, the barcodes are generated with a length of 650 bp using standardized Sanger sequencing, but with the introduction of high-throughput sequencing (HTS) methods new opportunities for sequencing are available. To use HTS for barcode collection and identification, the amplification of shorter fragments is preferred. Reference DNA midi-barcodes of wild bees were produced using the Illumina MiSeq as well as the Sanger method. Although DNA midi-barcodes derived from Illumina were comparatively shorter (418 bp), their sequences were coherent to the morphological assignment of species. The Illumina barcodes proved to be effective and dealt better with some general limitations of DNA barcoding.

Funding

The project was supported by Ma22 and the Austrian Federal Ministry of Education, Science and Research via a project associated to the Austrian Barcode of Life Initiative (ABOL) within the framework of the ‘Hochschulraum-Strukturmittel’ Funds.

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