Isolation and antiproliferative activity of chemical constituents from <i>Asystasia buettneri</i> Lindau

<p><i>N</i>-hexane and methanol extracts of <i>Asystasia buettneri</i> Lindau aerial parts exhibited antiproliferative activity on leukaemia blood carcinoma, K-562. Hexadecane (<b>1</b>), 1,3-propan-2-ol (9Z,12′Z,15″Z)-bis(doeicos-9,12,15-trienoate) (<b>2</b>), hydrocarbon, 2,3,3,10,23-pentamethyl tetraeicos-10,13,16-trien-1-ol (<b>3</b>), hexadecanoic acid (<b>4</b>) and taraxerol (<b>5</b>) were isolated from <i>n</i>-hexane extract; stigmasterol (<b>6</b>) and <i>(Z)</i>-9-octadecenoic acid (<b>7</b>) were isolated from ethyl acetate extract; while unsaturated hydrocarbons, octadecene (<b>8</b>), 8-methyl tetradec-6-ene (<b>9</b>) and 19-methyl eicos-1-ene (<b>10</b>), fatty acids, <i>(Z)</i>-5-hexadecenoic acid (<b>11</b>), 11,22-dimethyl ethyltrieicos-11-enoate (<b>12</b>) and taraxasterol (<b>13</b>) were isolated from methanol extract of the plant. Compounds <b>4, 5, 7, 11, 12</b> and <b>13</b> exhibited antiproliferative activity against K-562, while compounds <b>5, 6, 7</b> and <b>9</b> revealed antiproliferative activity by inhibiting hepatic liver (WRL68) cell lines.</p>