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Mechanistic studies on the reaction between glutathionylcobalamin and selenocysteine

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Version 2 2019-09-18, 11:17
Version 1 2019-01-21, 16:00
journal contribution
posted on 2019-09-18, 11:17 authored by Ilia A. Dereven’kov, Sergei V. Makarov

The reaction between glutathionylcobalamin (GSCbl), a complex of Co(III)-cobalamin with glutathione, and selenocysteine (Sec) was investigated using ultraviolet-visible (UV–vis) spectroscopy. The interaction results in the formation of cob(II)alamin and proceeds via two pathways: (i) a rapid formation of complex between GSCbl and Sec followed by the rate-determining substitution of glutathionyl-ligand by Sec and rapid electron-transfer from Se-atom to Co(III)-ion and (ii) a nucleophilic attack of Co(III)-S bond by Sec.

Funding

This work was supported by the Russian Science Foundation, Agreement No. 14-23-00204 P.

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