Ovarian cancer is the deadliest gynecologic cancer. The large-scale microRNA (miRNA) expression profiling and individual miRNA validation was performed to find potential novel biomarkers for ovarian cancer. The most consistent overexpression of miRs-200b-3p, 135 b-5p and 182-5p was found in both ascitic fluid and tumors and suggests their potential as oncogenes. miR-451a was consistently underexpressed so may be a tumor suppressor. Results were inconsistent for miR-204-5p, which was overexpressed in ascitic fluid but underexpressed in tumor tissue. miR-203a-3p was generally overexpressed but this failed to be proved in independent sample set in tissue validation.
Funding
LZ received institutional funding from Charles University, Prague (Progres Q28/LF1) and research support from the Avast Foundation (0F2016\2016020048, SSD2018\100022), CEZ Foundation (STE EPP 126_16) and ČEPS a.s. (1410001544, 1410002088). AH received institutional funding from Charles University, Prague (Progres Q25/LF1) and research support from the Ministry of Health of the Czech Republic (grant project RVO-VFN 64165). VW and LM received research support from the Ministry of Health of the Czech Republic (CZ-DRO FNBr 65269705).