Taylor & Francis Group
Browse
lpbb_a_1550657_sm3602.docx (13.38 kB)

Production, characterization, and powder preparation of quorum quenching acylase AiiO for pathogen control

Download (13.38 kB)
journal contribution
posted on 2019-02-03, 13:57 authored by Yue Li, Jing Zhao, Chunshan Quan, Liming Jin, Yongbin Xu, Ming Chen

Acylase AiiO is a novel quorum quenching enzyme with a broad substrate spectrum of acyl-homoserine lactones (AHLs) and has promising prospects in pathogen control. In this work, acylase AiiO production by a recombinant E. coli strain and its characterization were investigated; the acylase powder was further prepared and evaluated for effectiveness. A strategy of auto-induction combined with temperature regulation was developed to improve AiiO production. For the soluble AiiO protein in the cells, maximum production of 214.3 ± 9.4 mg/L was obtained in the fermenter. The purified acylase displayed an obvious AHL-degrading specific activity of 19.2 ± 0.56 U/mg. Sucrose, as the protective agent, maintained good stability of the acylase powder, in which the acylase remained 89.6 and 71.9% of its initial specific activity after storage at 4 °C for 3 and 6 months, respectively. The acylase powder could prominently decrease the expression levels of virulence-related factors of Pseudomonas aeruginosa. Based on the high-yield production and effective powder preparation, the quorum quenching acylase AiiO has the potential to be used in the clinical treatments of pathogenic infections.

Funding

This work was supported by the Young Science and Technology Star Project of Dalian, China [grant number 2017RQ150] and Project of Liaoning Provincial Natural Science Foundation [grant number 20170540063].

History