Reporting effective extraction methodology and chemical characterization of bioactive components of under explored <i>Platycladus orientalis</i> (L.) Franco from semi-arid climate

<p>The bioactive ingredients of many essential oils are complex and difficult to reproduce synthetically. In the present study, the influence of extraction method on relative extraction of bioactive compounds of essential oil of <i>Platycladus orientalis</i> (L.) Franco (<i>Thuja orientalis</i> L. is a synonym of it) leaves gathered from semi-arid climate was studied. A higher yield of essential oil was obtained by hydrodistillation (0.1%) as compared to steam distillation (0.07%) under newly optimized conditions. Initial analysis of these oils by TLC showed the presence of different polarity groups ranging from non-polar terpene hydrocarbons to polar terpenoid alcohols. GC-MS analysis revealed that major component of hydrodistilled essential oil of <i>Platycladus orientalis</i> (L.) Franco leaves was α-pinene (17.83%) and steam distilled essential oil contained α-cedrol (12.44%). The results obtained in the present study emphasize that suitable extraction technique should be used to obtain particular component of interest.</p>