Taylor & Francis Group
Browse
kccy_a_1754585_sm1775.docx (158.12 kB)

Rybp orchestrates spermatogenesis via regulating meiosis and sperm motility in mice

Download (158.12 kB)
journal contribution
posted on 2020-04-23, 12:38 authored by Qing Tian, Shi-Meng Guo, Shi-Ming Xie, Ying Yin, Li-Quan Zhou

Ring1 and Yin Yang 1-Binding Protein (RYBP) is a member of non-canonical polycomb repressive complex 1 to mediate monoubiquitination of histone H2A at lysine 119. It plays an important role in development, but its role in reproduction remains illusive. In this study, we used Rybp conditional knockout mouse model to genetically ablate Rybp in male germ cells. We found that Rybp deficiency during spermatogenesis led to smaller testes, loss of germline cells, disturbed meiosis, increased apoptosis of spermatocytes, decreased sperm motility, and reduced global H3K9me3, without impacting retrotransposon expression. Meanwhile, we depleted Rybp during oogenesis, but oocyte maturation and preimplantation development were normal. Our findings demonstrate that RYBP plays important roles in spermatogenesis through regulating meiosis and sperm motility.

Funding

This work was supported by the National Key R&D Program of China [2018YFC1004502]; National Key R&D Program of China [2018YFC1004001]; the Fundamental Research Funds for the Central Universities [2019kfyXKJC074]; the Foundation of NHC Key Laboratory of Male Reproduction and Genetics [KF201804]; the National Natural Science Foundation of China [NSFC 31771661].

History

Usage metrics

    Cell Cycle

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC