Synchronous degradation of phenol and aniline by Rhodococcus sp.strain PB-1entrapped in sodium alginate-bamboo charcoal-chitosan beads

The biodegradation of benzene series compounds is a difficult problem in environment pollution control, which is attributed to the deficiency of high efficiency bacteria and suitable embedding materials. In this study, the immobilized cells Rhodococcussp. strain PB-1 was used to synchronously biodegrade phenol and aniline by entrapped in sodium alginate (SA)-bamboo charcoal (BC)-chitosan acetate (CA) beads. The free cells of the strain PB-1 could completely degrade 1500 mg/L phenol or 800 mg/L aniline within 48 h, while the degradation rate of 2000 mg/L phenol and 1500 mg/L aniline was 35.76% and 68.06% at 72 h, respectively. The ortho-cleavage pathway was used to degrade phenol and aniline by strain PB-1. However, after entrapped with SA-BC-CA beads,the removal rate of 2000 mg/L phenol was 100% at 108 h, 1500 mg/L aniline was 100% at 62 h and 2000–3000 mg/L total toxic compounds was over 95% at 120 h. These beads could be used four times and were more effective than SA or SA-BC beads. The SA-BC-CA beads could remarkably improve the stability and degradation efficiency of strain PB-1, and thus provide a potential application in the removal of phenol and aniline in wastewater.