Validation of a 6 Dye Mini STR kit with 17 loci

The AGCU 6 Dye Mini STR kit is designed to amplify DNA from extracts as well as direct amplification from substrates. Through 6 dye (i.e. FAM, HEX, VIG550, VIG598, VIG618 and SIZ-500), this kit combines 15 autosomal STR loci (i.e. D3S1358, D13S317, D7S820, D16S539, D2S1338, TH01, D18S51, TPOX, vWA, D21S11, D8S1179, D5S818, CSF1PO, D19S433, FGA) with a Y-STR locus (DYS391) and Amelogenin.

Containing the most widely used autosomal STR loci, the new system can not only be well applied for actual cases, but also can help the establishment of STR database, especially for the amplification of degraded DNA or low-template DNA. According to the SWGDAM developmental validation guidelines, the new system was validated for the sensitivity detection, species specificity, inhibitor tolerance, DNA mixtures, casework samples, concordance studies. The results demonstrate that the AGCU 6 Dye Mini STR kit is an efficient and reliable amplification kit, which can be used for human identification testing.