In-silico interaction of RNA silencing suppressors of velvet bean severe mosaic virus with DICER domains

Velvet bean severe mosaic virus (VbSMV), a bipartite begomovirus causes severe mosaic, downward curling of the leaves, stunting of the plants in velvet beans (Mucuna pruriens). VbSMV contains two potential viral suppressors of RNA silencing, AC2 and AV2. To understand the mechanism of suppression of host defence and interaction of VSRs with host DICER, the in-silico interaction study was performed. Evidence of interaction of the AC2 protein with the type III restriction enzyme domain, helicase superfamily c-terminal domain and transcription repair coupling factor, whereas AV2 protein with ribonuclease III C terminal domain, ERCC4-related helicase domain, ribonuclease III domain, Dicer dimerization domain, type III restriction enzyme RES subunit and transcription repair coupling factor were found. This is the first in-silico evidence of interaction of any begomoviral VSRs (viral suppressors of RNA silencing) with Dicer of host.