Celastrol-based nanomedicine hydrogels eliminate posterior capsule opacification - Supplementary table

Aim: To formulate an injectable thermosensitive micelle–hydrogel hybrid system loaded with celastrol

(celastrol-loaded micelle hydrogel: CMG) to prevent posterior capsule opacification (PCO). Materials

& methods: Celastrol-loaded micelles were embedded in a thermosensitive hydrogel matrix to enable

controlled on-demand celastrol delivery into the residual capsule. The efficacy and mechanisms of the

system for eliminating PCO were evaluated in rabbits. Results: Celastrol-loaded micelles inhibited the

migration and proliferation of lens epithelial cells induced by TGF-β1. Celastrol prevents epithelial–

mesenchymal transition in lens epithelial cells induced by TGF-β1 through the TGF-β1/Smad2/3/TEAD1

signaling pathway. In vivo efficiency evaluations showed that CMG demonstrated an excellent inhibitory

effect on PCO in rabbits and had no obvious tissue toxicity. Conclusion: Injectable CMG may represent

a promising ophthalmic platform for preventing PCO. This versatile injectable micelle–hydrogel hybrid

represents a clinically relevant platform to achieve localized therapy and controlled release of drugs in

other disease therapies.