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Development of Fe3O4/reduced graphene oxide nanocomposite-based dispersive solid-phase extraction for the quantification of five steroid hormones: supplementary materials

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posted on 2023-05-01, 13:50 authored by Nese Ular Cagatay, Murat Emrah Mavis, Sezin Erarpat, Sezgin Bakırdere

Aim: An accurate and sensitive analytical method was proposed to detect some steroid hormones

in biological samples. Materials & methods: An Fe3O4/reduced graphene oxide nanocomposite-based

dispersive solid-phase extraction was developed for the effective and simple preconcentration of steroid

hormones from human serum samples. Results & conclusion: The nanocomposite was firstly used as

adsorbent to simultaneously extract the selected hormones. Limit of detection values for the selected

hormones were calculated between 5.5 and 39.2 ng/kg (mass based). An artificial serum sample was used

to test the applicability and accuracy of the developedmethod; percentage recovery results obtained from

two different spiked concentrations were found to be in the range of 80.5–99.9%.

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