Perspective on high-throughput bioanalysis to support in vitro assays in early drug discovery: Supplementary tables

<p>As the desire for a shortened design/make/test/learn cycle increases in early drug discovery, the</p> <p>pressure to rapidly deliver drug metabolism pharmacokinetic data continues to rise. From a bioanalytical</p> <p>standpoint, in vitro assays are challenging because they are amenable to automation and thus capable</p> <p>of generating a high number of samples for analysis. To keep up with analysis demands, automated</p> <p>method development workflows, rapid sample analysis approaches and efficient data analysis software</p> <p>must be utilized. This work provides an outline of how we implemented those three aspects to provide</p> <p>bioanalytical support for in vitro drug metabolism pharmacokinetic assays, which include developing</p> <p>hundreds of mass spectrometry methods and analyzing thousands of samples per week, while delivering</p> <p>a median bioanalytical turnaround time of 1–2 business days.</p>