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PolyIC-coated Prussian blue nanoparticles as a dual-mode HIV latency reversing agent - Supplementary dataset

dataset
posted on 2023-02-03, 11:41 authored by Preethi B Balakrishnan, Carissa S Holmberg, Debbie K Ledezma, Alberto Bosque, Rohan Fernandes

Aim: To investigate Prussian blue nanoparticles (PBNPs) coated with the synthetic analog of dsRNA

polyinosinic-polycytidylic acid (polyIC) for their ability to function as HIV latency reversing agents.

Methods: A layer-by-layer method was used to synthesize polyIC-coated PBNPs (polyIC-PBNPs). PolyICPBNPs

were stable and monodisperse, maintained the native absorbance properties of both polyIC and

PBNPs and were obtained with high nanoparticle collection yield and polyIC attachment efficiencies.

Results: PolyIC-PBNPs were more effective in reactivating latent HIV than free polyIC in a cell model of

HIV latency. Furthermore, polyIC-PBNPs were more effective in promoting immune activation than free

polyIC in CD4 and CD8 T cells. Conclusion: PBNPs function as efficient carriers of nucleic acids to directly

reverse HIV latency and enhance immune activation.

Funding

National Institute of Allergy and Infectious Diseases, (Grant / Award Number: 'AI117970','R01-AI124722','R33AI136102','T32 AI158105')

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