Prediction of frozen virus stability based on degradation mechanisms, real-time data and modeling: Supplementary tables
Aim: Critical virus reagents in regulated bioanalytical assays require stability monitoring. Although
stability at ultralow frozen temperatures is generally assumed, published data are limited and realtime
studies are time consuming. Materials & methods: The authors reviewed literature data, typical
mechanisms of molecular degradation, glass transition temperatures of commonly used buffers and
available real-time storage data to model frozen virus reagent stability. Results: Storage at ultralow
temperatures below the glass transition temperature was critical for virus stability. Modeling of real-time
data suggested that virus potency remained within 0.5 log10 of its starting potency at a probability of >99,
90 and 73% after 10, 20 and 30 years, respectively. Conclusion: The study supports the practice of virus
storage at -70◦C or below for 20–30 years.