Supplemental-File2_miRNA-dataser.xlsx (5.22 MB)
Supplementary File 2. High-throughput miRNA-sequencing of the human placenta: expression throughout gestation
dataset
posted on 2021-05-25, 08:46 authored by Tania L Gonzalez, Laura E Eisman, Nikhil V Joshi, Amy E Flowers, Di Wu, Yizhou Wang, Chintda Santiskulvong, Jie Tang, Rae A Buttle, Erica Sauro, Ekaterina L Clark, Rosemarie DiPentino, Caroline A Jefferies, Jessica L Chan, Yayu Lin, Yazhen Zhu, Yalda Afshar, Hsian-Rong Tseng, Kent Taylor, John Williams III, Margareta D PisarskaAim: To understand
miRNA changes across gestation in healthy human placentae. This is essential
before miRNAs can be used as biomarkers or prognostic indicators during
pregnancy. Materials & Methods: Using next-generation sequencing, we
characterize the normative human placenta miRNome in first (N=113) and third
trimester (N=47). Results and Conclusions: There are 801 miRNAs expressed in
both first and third trimester, including 182 with similar expression across
gestation (P≥0.05, FC≤2) and 180 significantly different (FDR<0.05,
FC>2). Of placenta-specific miRNA clusters, C14MC decreases across gestation
and C19MC is overall highly expressed. Chromosome 13 clusters are upregulated in
first trimester. This work provides a rich atlas of healthy pregnancies to
direct functional studies investigating the epigenetic differences in first and
third trimester placentae.
