Supplementary Material. The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer’s disease brain.

<table> <tr> <td> <p><b>Supplementary Material</b>. The histone modification H3K4me3 is altered at the ANK1 locus in Alzheimer’s disease brain.</p> <p> </p> <p><u>Background</u></p> <p>Several epigenome-wide association studies of DNA methylation have highlighted altered DNA methylation in the <i>ANK1 </i>gene in Alzheimer’s disease brain samples. However, no study has specifically examined <i>ANK1 </i>histone modifications in the disease.</p> <p> </p> <p><u>Methods</u></p> <p>We use chromatin immunoprecipitation-qPCR to quantify tri-methylation at histone 3 lysine 4 (H3K4me3) and 27 (H3K27me3) in the <i>ANK1</i> gene in entorhinal cortex from donors with high (N= 59) or low (N=29) Alzheimer’s pathology.</p> <p> </p> <p><u>Discussion</u></p> <p>We demonstrate decreased levels of H3K4me3, a marker of active gene transcription, with no change in H3K27me3, a marker of inactive genes. H3K4me3 is negatively correlated with DNA methylation in specific regions of the <i>ANK1 </i>gene.</p> <p> </p> <p><u>Conclusions</u></p> <p>Our study suggests that the <i>ANK1 </i>gene shows altered epigenetic marks indicative of reduced gene activation in Alzheimer’s disease.</p> <p> </p> </td> </tr> </table><br>