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Influence of C107R mutation from hepatitis B virus genotype H on in vitro hepatitis B surface antigen detection and IFN-β-1a treatment: Supplementary figures

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posted on 2022-10-28, 17:04 authored by Marina Campos-Valdez, Sina Feustel, Hugo Christian Monroy-Ram´ırez, Carolina Barrientos-Salcedo, Miriam Fabiola Ayon-Perez, Martha Eloısa Ramos-Marquez, David A Fernandez-Galindo, Jorge Antonio Silva-Gomez, Arturo Santos, Juan Armendariz-Borunda, Laura Veronica Sanchez-Orozco

Aim: Assess the in vitro effect of hepatitis B virus (HBV) genotype H (HBV/H) with the small surface HBV

protein (HBs) C107R mutation on hepatitis B surface antigen (HBsAg) detection, TGFB1, CAT and IFNB1A

expression, and the response to IFN-β-1a treatment. Methods: HBV/H wild-type and HBs C107R variant

replicons were constructed and transfected into hepatic stellate cells and/or Huh7 that were later

treated with IFN-β-1a. HBsAg, HBV-DNA, pgRNA, TGFB1, CAT and IFNB1A expression was analyzed.

3D HBs structure from wild-type and C107R were foreseen by AlphaFold protein predictor, and IFN-β-

1a antiviral effect was evaluated. Results: C107R mutation did not impact viral replication, but HBsAg

serologic detection was affected.Wild-type and C107R similarly modified gene expression and responded

to IFN-β-1a. Conclusion: C107R disrupts the Cys107/Cys138 disulfide bond and impairs HBsAg detection.

Independently of the mutation, there were changes in TGFB1, CAT and IFNB1A expression, and a medium

response to IFN-β-1a treatment compared with genotype A and C.

Funding

Consejo Nacional de Ciencia y Tecnolog´ıa (CONACYT), grant no. 169824. Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara. Fortalecimiento Institutos 2019 grant. M Campos-Valdez was part of the CONACYT doctoral fellowship program (no. 824914).

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