Taylor & Francis Group
Browse
IMAGE
Supplementary Figure 1.tif (5.2 MB)
IMAGE
Supplementary Figure 2.tif (879 kB)
IMAGE
Supplementary Figure 3.tif (637.75 kB)
IMAGE
Supplementary Figure 4.tif (954.97 kB)
IMAGE
Supplementary Figure 5.tif (497.23 kB)
1/0
5 files

Loop-mediated isothermal amplification assay in combination with lateral flow dipstick in HSV type 1: Supplementary figures

Download all (8.1 MB)
figure
posted on 2023-02-03, 12:41 authored by Fei Gao, Zhuoshi Wang, Mingqi Zhang, Yan Zhao, Tao Yao, Ling Xu, Xiangdong He, Bing Wang, Wei He

Aim: Diagnostic methods for HSV-1 need to be updated. Materials & methods: Serial dilutions of HSV-

1 DNA and ocular pathogenic microorganisms were tested by loop-mediated isothermal amplificationlateral

flow dipstick assay (LAMP-LFD assay). The reliability of the LAMP-LFD assay and quantitative realtime

PCR (qPCR) for HSV-1 detection was also validated using 30 clinical swab samples. Results: The LAMP

reaction is optimal at 65◦C and 30 min. And the LAMP-LFD assay could detect HSV-1 up to 10 copies/μl and

no cross-reactivity with other pathogens was observed. Moreover, LAMP-LFD had similar positive rates

to qPCR in clinical samples, and sensitivity and specificity were highly consistent with those of qPCR.

Conclusion: LAMP-LFD assay is a rapid technique for the diagnosis of HSV-1.

Funding

This work was supported by the Shenyang Science and Technology Project (no. 20-301-4-00) and Natural Science Funding of Liaoning Province (nos. 2019-ZD-0994 and 2020-BS-292).

History

Usage metrics

    Future Virology

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC