Promotional effects of HIF1α and KDM3A interaction on vascular smooth muscle cells in thoracic aortic dissection. Supplementary figures and tables
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Supplementary Figure 1 Effect of HIF1α on the proliferation and migration of MOVAS cells induced by Ang-Ⅱ. A, Representative images of EdU-positive MOVAS cells induced by Ang-Ⅱ in the absence of HIF1α. B, Representative images of migration of MOVAS cells induced by Ang-Ⅱ in the absence of HIF1α detected by scratch test. Supplementary Figure 2 Expression of HES1 and KDM3A in the TAD samples. A, Expression of HES1 in the normal and TAD samples in the GSE52093 dataset. B, Expression of KDM3A in the normal and TAD samples in the GSE52093 dataset. Supplementary Figure 3 Effect of HIF1α on the proliferation and migration of MOVAS cells induced by Ang-Ⅱ by regulating HES1. A, Representative images of EdU-positive MOVAS cells induced by Ang-Ⅱ following treatment with oeHIF1α, sh-HES1 or both. B, Representative images of migration of MOVAS cells induced by Ang-Ⅱ following treatment with oeHIF1α, sh-HES1 or both detected by scratch test. Supplementary Figure 4 Effect of KDM3A on the proliferation and migration of MOVAS cells induced by Ang-Ⅱ via the HIF1α/HES1 axis. A, Representative images of EdU-positive MOVAS cells induced by Ang-Ⅱ following treatment with oeKDM3A, oeHIF1α or both. B, Representative images of migration of MOVAS cells induced by Ang-Ⅱ following treatment with oeKDM3A, oeHIF1α or both detected by scratch test. Supplementary Figure 5 Expression of HIF1α, HES1 and KDM3A in the mouse aortic tissue and the histopathological changes of aortic tissues. A, Immunohistochemistry analysis of HIF1α, HES1 and KDM3A proteins in the aortic tissue of mice. B, EVG staining analysis of histopathological changes in the aortic tissue of mice. |
