posted on 2022-02-14, 08:53authored byLucy M. Kimbley, Rachel Parker, Jack Harrington, Robert C Walker, Ben L Grace, Jonathan WestJonathan West, Timothy J Underwood, Matthew J.J. Rose-Zerilli
Supplemental Figure 1: Pre-combined analysis. (A) UMAP clustering of
nuclei isolated by the EZ method with cell types identified. (B) UMI
count of EZ nuclei across clusters. (C) Gene count of EZ nuclei
stratified by cell type cluster. (D) UMAP clustering of nuclei
isolated by the citric acid method with cell types identified. (E) UMI
count of citric acid nuclei across clusters. (F) Gene count of citric
acid nuclei stratified by cell type cluster.
Funding
T.J.U is supported by a Royal College of Surgeons of England and Cancer Research UK Advanced Clinician Scientist Fellowship (A23924). R.C.W is supported by a Cancer Research UK Clinical Research Training Fellowship (A25162). Development of Drop-seq technology in Southampton was funded by MRC grant (MC_PC_15078). This work was supported by a grant from the Chan Zuckerberg Initiative (Human Cell Atlas Pilot project: START: Standardization of Single-Cell and Single-Nucleus RNA-Seq Protocols for Tumors) to J.J.W, T.J.U. and M.J.J.R-Z. and a grant from the Southampton CR UK Development Fund to J.J.W, T.J.U. and M.J.J.R-Z.