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Supplementary Figure 3. Silver nanoparticles induce apoptosis via NOX4-derived mitochondrial ROS and ER stress in colorectal cancer cells

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posted on 2021-05-20, 09:27 authored by Juan-Hua Quan, Fei Fei Gao, Jia-Qi Chu, Guang-Ho Cha, Jae-Min Yuk, Weiyun Wu, Young-Ha Lee

Supplementary Figure 3. AgNPs induced PARP cleavage through mitochondrial ROS in HCT116 cells. HCT116 cells were pretreated with or without 0.5 μM DPI for 4 h and incubated with 5 μg/ml AgNPs for 24 h. Representative images obtained by confocal fluorescence microscopy stained with cleaved PARP antibody. Cleaved PARP-positive cells were observed following the AgNP treatment while cleaved PARP-positive cells were apparently decreased in cells pretreated with DPI.

Funding

This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF-2019R1A2C1088346) at Chungnam National University, and the National Natural Science Foundation of China (81971389 and 81771612), the Characteristic Innovation Projects of Guangdong Universities (2018KTSCX079, 2018KTSCX081) and the Guangdong Basic and Applied Basic Research Foundation (2019A1515011888 and 2019A1515011715).

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    Nanomedicine

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    Keywords

    Silver nanoparticlesColorectal cancerReactive oxygen speciesApoptosisNOX4ER stressXenograft nude miceCancer Cell Biology

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