gbif_a_704366_sup_27426173.pdf (120.16 kB)
A green and bio-inspired process to afford durable anti-biofilm properties to stainless steel
journal contribution
posted on 2012-08-01, 00:00 authored by E. Faure, C. Vreuls, G. Zocchi, C. Van De Weerdt, J. Martial, C. Detrembleur, C. Falentin-Daudré, C. Jérôme, A.-S. DuwezA bio-inspired durable anti-biofilm coating was developed for industrial stainless steel (SS) surfaces. Two polymers inspired from the adhesive and cross-linking properties of mussels were designed and assembled from aqueous solutions onto SS surfaces to afford durable coatings. Trypsin, a commercially available broad spectrum serine protease, was grafted as the final active layer of the coating. Its proteolytic activity after long immersion periods was demonstrated against several substrata, viz. a synthetic molecule, N-α-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA), a protein, FTC-casein, and Gram-positive biofilm forming bacterium Staphylococcus epidermidis.