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A novel reversible fluorescent probe for Zinc(II) ion and bioimaging in living cells

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posted on 2020-04-09, 17:55 authored by Yongxin Chang, Bai Li, Huihui Mei, Kuxi Xu, Xinmei Xie, Li Yang

A simple Schiff-base fluorescent probe (L) was synthesised for selective detection of Zn2+ ion in CH3CN/HEPES solution (1/1, 10.0 μM, pH = 7.0). The probe L showed highly fluorescence enhancement towards Zn2+ over other metal ions with obvious fluorescence colour change from faint yellow to blue, which should be attributed to the formation of a 1:2 ligand-metal complex resulting in the inhibition of intramolecular charge transfer (ICT) and the chelation-enhanced fluorescence (CHEF) process. The bonding mechanism was confirmed by UV-vis, fluorescence, HRMS, 1 H NMR experiments and theoretical calculation. Reversibility of the probe in its binding with Zn2+ ion was established in the presence of EDTA. Furthermore, the fluorescence imaging indicated that probe L could be used to effectively identify Zn2+ in living cells.

A novel simple Schiff-base fluorescent probe (L) has been prepared for selective detection of Zn2+ ion in CH3CN/HEPES solution (1/1, 10.0 μM HEPES, pH 7.0). The probe L showed highly fluorescence enhancement to Zn2+ over other metal ions with obvious fluorescence colour change from colourless to yellow, which might be attributed to the formation of a 1:2 ligand-metal complex resulting in the inhibition of intramolecular charge transfer (ICT) and the chelation-enhanced fluorescence (CHEF) process. The bonding mechanism was confirmed by UV-vis, fluorescence, 1H NMR experiment and theoretical calculation. Reversibility of the probe in its binding with Zn2+ ion is established in the presence of EDTA. Furthermore, the fluorescence imaging indicated that probe L could be used to effectively identify Zn2+ in living cells.

Funding

This work was supported by the the National Natural Science Foundation of China [31872311].

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