Anti-inflammatory and antioxidant activities of Citrus aurantifolia (Christm.) Swingle essential oil in dystrophic muscle cells: implication of the PGC-1α pathway

dos Santos Rapucci Moraes, Fernanda; Moraes, Luis Henrique Rapucci; Macedo, Aline Barbosa; Valduga, Amanda Harduim; Mizobuti, Daniela Sayuri; da Rocha, Guilherme Luiz; da Silva, Heloina Nathalliê Mariano; Salvador, Marcos José; Minatel, Elaine

doi:10.6084/m9.figshare.24947341.v1

teop_a_2296994_sm4044.pdf (258.77 kB)

Anti-inflammatory and antioxidant activities of Citrus aurantifolia (Christm.) Swingle essential oil in dystrophic muscle cells: implication of the PGC-1α pathway

journal contribution

posted on 2024-01-05, 05:21 authored by Fernanda dos Santos Rapucci Moraes, Luis Henrique Rapucci Moraes, Aline Barbosa Macedo, Amanda Harduim Valduga, Daniela Sayuri Mizobuti, Guilherme Luiz da Rocha, Heloina Nathalliê Mariano da Silva, Marcos José Salvador, Elaine Minatel

The commercial essential oil obtained from the steam distillation of cold-pressed oil from Citrus aurantifolia (Christm.) Swingle fruits (OCA), after chemical characterization by gas chromatography-mass spectrometry (GC-MS), was investigated for antioxidant and anti-inflammatory activities and related signaling pathways in dystrophic muscle cells. The chemical composition of OCA showed 24 compounds with a predominance of monoterpenes, such as limonene (42.6%), cymene (11.8%), α-terpineol (11.8%), terpinolene (5.3%) and γ-terpinene (4.6%). DPHH and ORAC assays showed a significant antioxidant activity of OCA. Because the OCA is rich in limonene, it exhibited marked anti-inflammatory (as indicated by the reduction of TNF- α and NF-κB levels) and antioxidant effects (as indicated by the reduction of 4-HNE-protein adducts, SOD-2, GSR and H₂O₂ levels) on dystrophic muscle cells. These effects appear to be mediated through the activation of PGC-1α pathway. Therefore, OCA can be considered a promising bioactive for therapeutic applications in dystrophinopathies.